Dynamic genome-wide mapping reveals how chromatin context shapes OGG1-mediated repair and related mutagenesis in human cells.

ABSTRACT: Potassium bromate-induced DNA damage, including 8-oxo-7,8-dihydroguanine (OG) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG), are common oxidatively generated DNA lesions with mutagenic potential if not efficiently repaired. While sequencing-based studies have shown that damage formation is influenced by DNA sequence context, secondary structures, and chromatin features, how OGG1-mediated repair is regulated within chromatin remains unclear. Here, we apply CLAPS-seq to generate genome-wide, single-nucleotide resolution maps of OGG1-mediated repair over time in human cells, and systematically analyze how chromatin context affects repair efficiency across hierarchical scales. We find that chromatin accessibility governs rapid initial repair, whereas higher-order chromatin structures

SUBMITTER: Li J 

PROVIDER: S-EPMC12684389 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature