Telomeric DNA damage landscapes distinguish oxidative from inflammatory cellular stress.

ABSTRACT: Telomere dysfunction and attrition are observed in cells subjected to oxidative or inflammatory stress, largely due to DNA damage. In this study, we applied a DNA glycosylase-assisted quantitative PCR (qPCR) assay to assess telomeric DNA damage in human cells exposed to defined sources of oxidative or inflammatory stress. As a consequence of the endogenous chemistry of these stressors being different, we were able to identify distinct types of telomere DNA damage that differentiate oxidative from inflammatory stress. By selecting lesion-specific DNA glycosylases before qPCR analysis, we determined that reactive oxygen species generated under physiological bicarbonate buffering during both oxidative and inflammatory stress primarily damaged 2'-deoxyguanosine (dG) residues. In contrast, infl

SUBMITTER: Fleming AM 

PROVIDER: S-EPMC12684392 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature