Circulating Cathepsin D Exacerbates Injury-Induced Brain Damage by Promoting Neutrophil Infiltration Into the Brain.

ABSTRACT:

Background

Communication between the vascular endothelium and peripheral leukocytes is an important process controlling inflammatory responses in the brain. Increased plasma levels of CTSD (cathepsin D) have been associated with vascular events, but whether circulating CTSD may affect the functional status of the endothelium remains unknown.

Methods

To resolve this issue, two groups of transgenic hCTSD^hi mice with high levels of circulating CTSD were subjected to traumatic and ischemic injury, respectively. Then, single-cell RNA sequencing was performed to characterize gene expression changes in the brains of hCTSD^hi mice. Additionally, transgenic CTSD^{Mono knockout} mice with reduced plasma CTSD levels were generated to investigate whether CTS

SUBMITTER: Liang Y 

PROVIDER: S-EPMC12684490 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature