Multiomics Analysis of Skeletal Muscle Identifies Dysregulation of Hypoxia-Induced Genes in Peripheral Artery Disease.

ABSTRACT:

Background

Epigenetic modifications such as DNA methylation play a critical role in hypoxic cell programs. However, no previous studies have investigated the epigenetic regulation of gene expression in peripheral artery disease (PAD), a condition characterized by intermittent ischemia. Our study aims to examine how PAD affects the DNA methylome in skeletal muscle of patients with PAD with intermittent claudication or critical limb ischemia compared with non-PAD controls.

Methods

This was a cross-sectional study evaluating differences in the DNA methylome and transcriptome using reduced representation bisulfite sequencing, and small and bulk RNA sequencing, in skeletal muscle from non-PAD controls, intermittent claudication, and critical limb ischemia. We also assessed change

SUBMITTER: Ismaeel A 

PROVIDER: S-EPMC12684623 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature