Investigating a new <i>Dillenia ovata</i> endophytic bacteria to produce antioxidants and anti-diabetes activity <i>in vitro</i> and <i>in vivo</i>.

ABSTRACT: A novel endophytic bacterium, Bacillus sp. DO-R5, with strong antioxidant and anti-diabetic properties, was isolated from Dillenia ovata, a traditional Vietnamese medicinal plant. Bacillus sp. DO-R5 was identified using morphological characteristics, SEM, and 16S rRNA sequencing, demonstrated the highest bioactivity among the 19 isolates. Optimal fermentation conditions (8.9 g/L D-glucose, pH 7.5, 38 °C, and 70.5 h) were determined using the Box-Behnken design. Under these conditions, Bacillus sp. DO-R5 significantly enhanced the production of bioactive compounds including diosmin, rutin, and chlorogenic acid, and exhibited improved in vitro antioxidant, anti-inflammatory, and anti-diabetic activities. In vivo, the extract effectively reduce

SUBMITTER: Tran LC 

PROVIDER: S-EPMC12684764 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature