Comprehensive multi-omics analyses expose a precision therapy strategy that targets replication stress in hepatocellular carcinoma using WEE1 inhibition.

ABSTRACT:

Introduction

Hepatocellular carcinoma (HCC) is an extremely heterogeneous malignancy with a poor prognosis, highlighting the need to target specific vulnerabilities within the tumor during treatment.

Objectives

This study employs multi-omics analysis techniques to provide novel insights into personalized therapeutic strategies for HCC patients.

Methods

We performed proteomic and transcriptomic sequencing on 178 and 94 clinical samples of primary HCC without prior treatment, respectively. We employed an unbiased Kinome CRISPR-Cas9 library screening approach to systematically evaluate and identify novel therapeutic strategies that specifically target replication stress (RS). The synergy between oxaliplatin and adavosertib was verified using in vitro and in vivo models,

SUBMITTER: Jia X 

PROVIDER: S-EPMC12684918 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature