Synthetic tunable promoters for flexible control of multi-gene expression in mammalian cells.

ABSTRACT:

Introduction

Synthetic biology revolutionizes our ability to decode and recode genetic systems. The capability to reconstruct and flexibly manipulate multi-gene systems is critical for understanding cellular behaviors and has significant applications in therapeutics.

Objectives

This study aims to construct a diverse library of synthetic tunable promoters (STPs) to enable flexible control of multi-gene expression in mammalian cells.

Methods

We designed and constructed synthetic tunable promoters (STPs) that incorporate both a universal activation site (UAS) and a specific activation site (SAS), enabling multi-level expression control via the CRISPR activation (CRISPRa) system. To evaluate promoter activity, we utilized Massively Parallel Reporter Assays (MPRA) to asses

SUBMITTER: Fu ZH 

PROVIDER: S-EPMC12684930 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature