Factors affecting the intracytoplasmic sperm cell injection outcomes: A meta-analysis of porcine studies.

ABSTRACT:

Background

Intracytoplasmic sperm cell injection (ICSI) has the potential to produce gene-edited (GE) pigs for biomedical research, but its success is limited. The factors impeding ICSI in pigs are impractical in-vivo oocyte production, incomplete cytoplasmic maturation of in-vitro matured (IVM) oocytes, inefficient methods for sperm selection and membrane removal, abnormal sperm nucleus decondensation, substandard protocols for oocyte stimulation, suboptimal in-vitro culture (IVC) systems, and high embryonic/fetal losses.

Aim of review

The aim of this review is to investigate the effects of interventions in ICSI on oocyte activation, fertilization, cleavage, blastocyst, blastomere count, and live birth by means of robust statistical meta-analytical methods.

Key scientifi

SUBMITTER: Jamal MA 

PROVIDER: S-EPMC12684963 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature