Probing direct interactions between nuclear proteins in cells with nxReLo.

ABSTRACT: Nearly all biological processes depend on protein-protein interactions (PPIs). While various methods exist to study these interactions, investigating those that involve nuclear proteins, including structurally complex proteins containing long disordered regions, remains a significant challenge. Here, we developed nxReLo, a simple and fast cell culture-based colocalization assay, designed to identify and characterize interactions between nuclear proteins. PIWI-interacting RNAs (piRNAs) safeguard germline genome integrity, and the PPIs that facilitate piRNA production are therefore essential for animal reproduction, yet remain incompletely understood. We used nxReLo to investigate interactions between members of the Drosophila melanogaster Rhino-Deadlock-Cutoff (RDC) complex an

SUBMITTER: Ismail S 

PROVIDER: S-EPMC12685051 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature