Lipidomic profiling of endometrial cancer using desorption electrospray ionization mass spectrometry imaging.

ABSTRACT: Novel technologies are required to improve endometrial cancer diagnosis and enhance the early detection of preinvasive precursors. Herein, desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was used to differentiate malignant from benign endometrial tissues and assess lipidomic differences among patients with obesity and diabetes. Reverse phase protein array (RPPA) analysis was performed in the same patients to gain insights into the altered signaling pathways and investigate the protein expression levels in endometrial cancer cases and controls. Tissues from 64 women (50 cancer, 14 benign) were analyzed with DESI-MSI achieving 90% sensitivity and 93% specificity. Discriminatory spectral features were primarily phospholipids [phosphatidic acid (PA), phosphatidylethanol

SUBMITTER: Paraskevaidi M 

PROVIDER: S-EPMC12685106 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature