Emergence of endothelial subtypes and role of cell cycle control in arterial-venous specification during embryonic vascular development.

ABSTRACT: During vascular development, endothelial cells (ECs) specify into arterial, capillary, and venous subtypes to form a circulatory network. While the cell cycle state enables postnatal arterial-venous specification in a flow- and tissue-specific manner, its role during embryogenesis remains unclear. To investigate this, we isolated ECs at embryonic day (E)8.0 (pre-flow), E8.5 (post-flow), and E9.5 and performed single-cell RNA sequencing. Arterial, venous, and hemogenic subtypes emerged with significant enrichment of cell-cycle-related pathways. Using Fucci embryos, ECs were sorted into early G1, late G1, and S/G2/M states and profiled by bulk RNA sequencing. Integration with our single-cell data showed that venous ECs aligned with early G1 and arterial ECs aligned with late G1 transcription

SUBMITTER: Aragon JW 

PROVIDER: S-EPMC12685131 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature