Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography.

ABSTRACT: Cryoelectron tomography (cryo-ET) enables three-dimensional visualization of molecular structures within tissue and intact cells, providing a powerful tool for studying the spatial organization of biological components at nanometer resolution. Realizing this potential, particularly for submegadalton complexes, is facilitated by fiducial-based labeling. Gold nanoparticles (AuNPs) have emerged as powerful electron-dense labels for cryo-ET, but multiplexing using only conventional monomeric AuNPs is challenging, limiting their application in multitarget studies. Here, we describe functionalized dimeric AuNPs with a precisely defined size range applied to bioimaging, enabling multiplexed labeling by allowing reliable discrimination between monomeric and dimeric AuNPs, thereby supporting identi

SUBMITTER: Kim H 

PROVIDER: S-EPMC12685141 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature