Optical tissue clearing and 3D imaging of intact primate testicular tissue: a novel technology development.

ABSTRACT: Classical histology struggles to preserve three-dimensional spatial context, prompting the emergence of optical tissue clearing techniques that enable imaging of intact specimens at cellular or subcellular resolution. These techniques have revolutionised fields like cell biology, developmental biology, and neuroscience. However, their application in reproductive biology remains unexplored - particularly in studying the complexities of testicular development. We developed a novel, efficient and affordable toolbox for studying intact testicular tissues, PT-CLEAR3D, that stands for primate testis - whole mount staining, tissue clearing and three-dimensional imaging. Intact testicular tissues from humans (transgender model), common marmosets and macaques underwent antibody labelling, clearing

SUBMITTER: Kibui PW 

PROVIDER: S-EPMC12685185 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature