Multiepitope fusion protein-based ELISA for enhanced brucellosis serodiagnosis.

ABSTRACT:

Background

Brucellosis is a widespread zoonotic disease with approximately 2.1 million new human cases annually. Traditional serological methods, which rely on lipopolysaccharide (LPS) as a diagnostic antigen, suffer from cross-reactivity with other Gram-negative bacteria. To address these issues, we aimed to develop a multiepitope fusion protein for improved brucellosis diagnosis.

Methods

We identified linear B-cell epitopes from Brucella proteins using the Immune Epitope Database (IEDB) and constructed a multiepitope fusion protein. The fusion protein was expressed through prokaryotic expression, purified, and was evaluated using an indirect enzyme-linked immunosorbent assay (iELISA) with serum samples from patients with confirmed brucellosis (n = 279) and negative control

SUBMITTER: Zhan T 

PROVIDER: S-EPMC12685213 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature