Mechanistic study of <i>TFE3</i> breakage in <i>TFE3</i>-rearranged renal cell carcinoma: the perspective of non-canonical DNA structures and their stability.

ABSTRACT:

Background/objectives

To address the unelucidated mechanisms of breakpoint formation in TFE3-rearranged renal cell carcinoma (TFE3-rRCC), this study characterizes breakpoint distribution within the TFE3 gene. We further explore how non-canonical DNA structures and their thermodynamic stability fluctuation may act as predisposing factors for the genomic instability driving these characteristic translocations.

Methods

TFE3 breakpoints were identified in a cohort of 31 TFE3-rRCC tumor samples. The chi-square test was used to assess the statistical significance of breakpoint clustering. To investigate potential structural determinants, we predicted the distribution of G-quadruplex-forming sequences and palindromic motifs. Moving beyond simple m

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12685264 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature