Slo1 Deficient Myoblast Exosomes-Derived miR-222-3p Inhibits Osteogenic Differentiation via Targeting of STAT3.

ABSTRACT:

Background

Previous research has shown that the conditional knockout of Slo1 in muscle leads to a reduction in muscle strength and inhibits myogenic differentiation. Exosomes are emerging as necessary mediators of the crosstalk between muscle and bones. The present study focused on the communication between muscle and bone to investigate the effects and mechanisms of skeletal muscle-specific knockout of Slo1 on bone mass and bone metabolism.

Methods

Myf5-Cre mice were mated with Slo1^flox/flox mice to construct skeletal muscle-specific Slo1 knockout mice (CKO mice). The legs of the Slo1 CKO mice were isolated, and a series of examinations were conducted to monitor bone mineral density (BMD) and bone microstructure. Exosomes were extracted from C2C12-shNC and C2C12-

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12685407 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature