Auxin affects gene editing efficiency through regulating chromatin accessibility and plant regeneration process.

ABSTRACT: Improving gene editing efficiency has been a prominent research focus with the increasing application of CRISPR/Cas9 in crop genetic enhancement. In this study, we demonstrated that increasing exogenous auxin levels during in vitro tissue culture significantly enhances gene editing efficiency, leading to a higher frequency of functionally edited T₀ plants. While higher auxin levels promoted callus growth, it also delayed shoot initiation and slightly decreased shoot regeneration. Subsequent RNA-Seq analysis revealed significant alterations in the expression of plant developmental regulatory genes and chromatin remodeling genes at two plant regeneration stages. Further analysis using nuclei staining and Transposase-Accessible Chromatin using sequencing showed that excessiv

SUBMITTER: Lian Z 

PROVIDER: S-EPMC12685441 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature