Palmitic acid differently modulates extracellular vesicles and cellular fatty acid composition of SGBS adipocytes without impairing their insulin signaling.

ABSTRACT:

Scope

Adipocyte-derived extracellular vesicle (EV) lipid cargo reflects the obese metabolic state. Nevertheless, it is currently unknown whether the adipocyte-derived EV lipid profile is influenced by saturated fatty acid overload. This study investigated if palmitic acid (PA) affected human Simpson-Golabi-Behmel syndrome (SGBS) adipocyte insulin sensitivity and the repercussions on the EV fatty acid cargo secreted by these cells.

Methods

Adipocytes were treated with 500 or 1,000 μM of PA, and cytotoxicity was assessed using the lactate dehydrogenase assay. Thereafter, cells were treated with 1,000 μM of PA for 48 h, followed by the assessment of triglyceride accumulation and adipokine expression. Insulin signaling, NF-κβ activation, and stearoyl-CoA desaturase-1 (SCD) abund

SUBMITTER: Sergi D 

PROVIDER: S-EPMC12685657 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature