Pharmacological modulation of circadian rhythms in brain microvasculature.

ABSTRACT: The interface between the blood and brain is vital for the protection of neurological processes. A key regulatory mechanism of the blood-brain border (BBB) is the circadian rhythm, an intrinsic timekeeping system that maintains 24-h rhythms. At the BBB, the endothelial cell clock has been implicated in time-of-day differences in removal of drugs from the brain; however, pharmacologic modulation of the BBB clock remains unexplored. To investigate circadian dynamics in the BBB, we isolated murine brain microvessels (MVs) and used a Color-Switch PER2::iLuc system to longitudinally monitor rhythms. Isolated MVs consisting of endothelial cells and pericytes preserved vascular morphology for 4 days and circadian oscillations were maintained ex vivo under forskolin synchronization. MVs were treat

SUBMITTER: Yang Z 

PROVIDER: S-EPMC12685704 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature