Adaptive laboratory evolution enhances cell yield and lipid production of <i>Chlorella sorokiniana</i> under mildly cold conditions.

ABSTRACT: One challenge to large-scale microalgae cultivation, e.g., for biodiesel production, is the seasonal low-temperature conditions. We argue that seasonally varying selection in natural environments has prevented algae from better adapting to cold temperatures, and that laboratory evolution offers a promising approach for obtaining cold-adapted algal materials. We conducted a population-level artificial selection experiment with the unicellular green microalgae Chlorella sorokiniana at both a benign temperature (25 °C) and a mildly cold temperature (15 °C). Four artificial selection regimes were established: random selection, selection for high biomass (i.e., cell yield), selection for high lipid production, and rotation between high-biomass and high-lipid selection. The selection expe

SUBMITTER: Lu SY 

PROVIDER: S-EPMC12685799 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature