Oxidative stress-regulatory role of miR-10b-5p in the diabetic human cornea revealed through integrated multi-omics analysis.

ABSTRACT:

Aims/hypothesis

Prominent features of diabetic corneal disease are oxidative stress, neuropathy and epitheliopathy including delayed wound healing and dysfunction of limbal epithelial stem cells. We hypothesised that regulatory miRNAs altered in the diabetic cornea, such as miR-10b-5p, may be responsible for these abnormalities. We aimed to understand the molecular impact of miR-10b-5p increase in human diabetic vs non-diabetic limbal epithelial cells (LECs) enriched in limbal epithelial stem cells by identifying its target genes and proteins and testing it as a potential therapy for inhibiting oxidative stress in diabetic corneas.

Methods

LECs were isolated from diabetic and non-diabetic human autopsy corneas. Telomerase-immortalised human corneal epithelial cells (HCECs),

SUBMITTER: Zha D 

PROVIDER: S-EPMC12686106 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature