Universal Microfluidic Platform for Multifunctional Surface Modification of Small Extracellular Vesicles.

ABSTRACT: Small Extracellular vesicles (sEVs) hold great promise as therapeutic delivery vehicles due to their inherent biocompatibility. However, their clinical translation is limited by donor cell source dependency and inadequate targeting capabilities. To overcome these challenges, we introduce a universal surface engineering strategy that integrates lipid membrane anchoring with targeted ligand conjugation. At the core of this approach is the sEV Surface-Engineering microfluidic device (ExoSE), a dual-functional platform combining nanofluidic and microfluidic architectures. ExoSE consists of two interconnected modules: (1) a loading module that employs mechanoporation via nanochannels to transiently generate pores in sEV membranes, enabling highly efficient insertion of functionalized lipids and

SUBMITTER: Hong Y 

PROVIDER: S-EPMC12686133 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature