LINC02081 as an oncogene promotes cell proliferation and G2/M cycle through p53 signaling pathway in lung adenocarcinoma.

ABSTRACT:

Background

LINC02081 is a newly identified long non-coding RNA (lncRNA) with an uncharacterized biological function. This study aimed to investigate the role of LINC02081 in lung adenocarcinoma (LUAD).

Methods

Bioinformatics analysis utilizing The Cancer Genome Atlas (TCGA) database assessed LINC02081 expression and its clinical significance. Nuclear and cytoplasmic RNA fractionation detected the subcellular localization of LINC02081. Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8) assays, 5-ethynyl-2'-deoxyuridine (EdU) assays, and colony formation assays. Flow cytometry analyzed the cell cycle and apoptosis. p53 expression was assessed via immunofluorescence, while p21 messenger RNA (mRNA) levels were analyzed by reverse transcription quantitative polyme

SUBMITTER: Zeng L 

PROVIDER: S-EPMC12686211 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature