Macromolecular crystallography for mammalian body temperature in support of molecular biophysics methods.

ABSTRACT: This review describes various molecular biophysics methods (crystallography, mass spectrometry, NMR spectroscopy, electron cryo-microscopy (cryoEM), free electron laser and X-ray photon correlation spectroscopy) which can be used to investigate the molecular structure of proteins under diverse conditions (visually summarised in the thumbnail image for the journal contents pages). We focus particularly on those which permit for the incubation and/or experimental sample analysis at mammalian body temperature (37 °C) or at physiological conditions for yet higher temperatures such as thermophiles. Crystallography, a leading method in structure elucidation, in recent decades has been dominated by structures analysed at cryogenic temperatures to ensure best resolution and crystal stability under

SUBMITTER: Brink A 

PROVIDER: S-EPMC12686291 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature