Structural Determination of Bl-3, an Insecticidal Peptide from the <i>Buthacus leptochelys</i> Scorpion Venom.

ABSTRACT: Scorpion venoms contain a variety of peptides that exhibit toxicity toward insects or mammals by acting on ion channels. We previously isolated four insecticidal peptides (Bl-1, Bl-2, Bl-3, and Bl-4) from the venom of Buthacus leptochelys. Among these, the complete amino acid sequence of Bl-1 was determined, whereas only N-terminal partial sequences were obtained for the others. In the present study, we determined the complete sequence of Bl-3 through de novo sequencing of enzymatically digested fragments. The discrimination between Leu and Ile was achieved based on side-chain fragmentation observed under high-energy collision-induced dissociation conditions. Bl-3 was identified as a 65-residue peptide containing four disulfide bonds. During the sequencing analysis, deamidati

SUBMITTER: Shimase R 

PROVIDER: S-EPMC12686336 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature