Single-cell transcriptomics of the myeloid milieu reveals an angiogenic niche in triple-negative breast cancer.

ABSTRACT: Intratumoral myeloid cells are highly heterogeneous in terms of development and function and are pivotal for forming and regulating the tumor microenvironment. However, the myeloid milieu in triple-negative breast cancer (TNBC) remains poorly understood. Here, to elucidate this myeloid milieu, we integrated in-house and public single-cell RNA sequencing data. We detected diverse neutrophil and mononuclear-phagocyte subtypes and delineated their developmental trajectories and functions. Of particular interest were the VEGFA^hi neutrophil and SPP1^hi macrophage subtypes, which displayed protumoral functions, including angiogenesis. Spatial transcriptomics revealed that they colocalized with epithelial cancer cells and APLN^hi endothelial tip cells in a hypoxic r

SUBMITTER: Choi Y 

PROVIDER: S-EPMC12686459 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature