(Non)canonical Wnt signaling, cytoarchitecture and stemness: new insights from primary nonmetastatic, primary metastatic, regional and distant metastatic models of adrenocortical carcinoma.

ABSTRACT: Tumor cells rearrange cytoskeletal networks, harmonize signaling pathways and modulate cell fate to initiate tumor growth and metastasis. Activating mutations in the canonical Wnt-β-catenin pathway are associated with aggressiveness and poor prognosis in adrenocortical carcinoma (ACC). However, the contribution of noncanonical Wnt pathways, which are known to regulate cell polarity and migration, is still widely unknown. Here we comprehensively investigated canonical and noncanonical Wnt signaling along with cytoskeleton networks, stemness programs and HOX gene expression in recently developed 2D and 3D cultures of primary, local and distant metastatic ACC models. We report that tumor spheroids derived from nonmetastatic primary tumors correlated strongly with the overexpression of canonic

SUBMITTER: Shapiro I 

PROVIDER: S-EPMC12686465 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature