Single-Cell RNA Sequencing Identifies Accumulation of Fcgr2b + Virtual Memory-Like CD8 T Cells With Cytotoxic and Inflammatory Potential in Aged Mouse White Adipose Tissue.

ABSTRACT: Aging and obesity are associated with pro-inflammatory changes in adipose tissue. Overlapping mechanisms, such as the infiltration of inflammatory macrophages and T cells into visceral adipose tissue, have been implicated in contributing to inflammation. However, a comparative analysis of both states is needed to identify distinct regulatory targets. Here, we performed single-cell RNA sequencing of stromal vascular fractions (SVF) isolated from gonadal white adipose tissue (gWAT) of young mice fed either a normal or a high-fat diet, and aged mice fed a normal diet. Our analysis revealed that physiological aging, compared to high-fat diet-induced obesity, was associated with an accumulation of phenotypically distinct CD8 T cells resembling virtual memory (VM) CD8 T cells. These cells expres

SUBMITTER: Kumar A 

PROVIDER: S-EPMC12686556 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature