An Ad Libitum-Fed Diet That Matches the Beneficial Lifespan Effects of Caloric Restriction but Acts via Opposite Effects on the Energy-Splicing Axis.

ABSTRACT: Caloric restriction (CR) with fasting extends lifespan but is difficult to maintain in humans. Here, we compared conventional CR with periods of fasting to an ad libitum-fed low-protein, high-carbohydrate (LPHC) diet diluted 25% with non-digestible fibre. Both approaches similarly enhanced longevity and metabolic health in mice relative to a control diet. Proteomic analysis of liver tissue revealed that CR increased proteins associated with energy and mitochondrial pathways. By contrast, the LPHC diet reduced these pathways but increased the abundance of proteins associated with RNA metabolism and spliceosome pathways. These results for LPHC support the "energy-splicing resilience" axis theory of ageing. Our results suggest that ad libitum-fed diets can be designed to replicate, and potent

SUBMITTER: Brandon AE 

PROVIDER: S-EPMC12686583 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature