Resistance to doxorubicin-induced proteinuria and proteolytic activation of ENaC in 129S2/SvPas mice.

ABSTRACT: Doxorubicin treatment of mice represents a convenient model to study the effects of proteinuria on proteolytic processing of the epithelial Na⁺ channel (ENaC) and urinary Na⁺ and fluid handling. Prior studies have shown enhanced ENaC γ subunit proteolysis and Na⁺ and fluid retention in 129S1/SvImJ mice treated with doxorubicin. We examined whether 129S2/SvPas mice could be used to study doxorubicin-induced proteinuria. 129S2/SvPas mice treated with 18 μg/g doxorubicin exhibited significantly reduced urinary albumin/creatinine compared to that described for 129S1/SvImJ mice. Proteolytic processing of ENaC's γ subunit and sensitivity of urinary Na⁺ and K⁺ to the ENaC blocker, benzamil, were not enhanced by doxorubicin. Differences in the

SUBMITTER: Ray EC 

PROVIDER: S-EPMC12686598 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature