Model-guided chemical environment and metabolic network design to couple pathways with cell fitness.

ABSTRACT: Heterologous compound production is a complex trait since the native metabolic fluxes supplying the precursors, redox power, and energy are under multilevel cellular regulation. Improving complex traits using targeted engineering needs combinatorially charting the complex genetic underpinnings. While this is laborious, adaptive laboratory evolution (ALE) has been used to improve many traits of microbial strains that are of application relevance such as tolerance of harsh conditions and nutrient utilization. However, in contrast to such traits, heterologous production can seldom be intuitively coupled with cellular fitness. Here, a novel method EvolveXGA was developed for genome-scale metabolic model guided design of strategies combining chemical environments and genetic engineering of the

SUBMITTER: Kakko von Koch N 

PROVIDER: S-EPMC12686821 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature