Glomeruli of the Mouse Olfactory Bulb: Numbers, Sizes, Shapes.

ABSTRACT: The discovery of olfactory bulb glomeruli was reported by the neuroanatomist Camillo Golgi in 1875, but the number of glomeruli in a mammalian olfactory bulb remains unknown. The 1141 intact odorant receptor genes in the mouse genome are key determinants of the coalescence of axons of olfactory sensory neurons into glomeruli at recognizable positions in the olfactory bulb. Here, we devised a methodology for quantifying the numbers, sizes, and shapes of the glomeruli of mouse olfactory bulbs. Using serial two-photon tomography, we generated complete image stacks of 12 mouse olfactory bulbs labeled immunohistochemically as whole mounts with antibodies against the glomerular marker VGLUT2. The mouse ages were postnatal days 14, 21, and 56. We manually segmented 33,137 glomeruli based on the V

SUBMITTER: Weng Y 

PROVIDER: S-EPMC12686850 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature