Ultrasonic frequency and intensity dependence in β₂-microglobulin amyloid seed detection: Balancing cavitation-induced nucleation, fragmentation, and radical formation.

ABSTRACT: Amyloid fibrils are aberrant proteinaceous aggregates involved in several intractable diseases called amyloidosis. Detecting fragments of amyloid fibrils, so-called seeds, in human biofluids is a promising approach for their early-stage diagnosis. Ultrasonication of amyloidogenic protein solutions has the potential to enhance the detection sensitivity of amyloid seeds in an accelerated manner through the effects of ultrasonic cavitation. However, the effects of acoustic frequency and intensity on ultrasonic seed detection have not been investigated. In this study, we explore the optimized acoustic conditions for rapid and sensitive amyloid-seed detection and sonochemical mechanisms behind it. Our results show that maximum detection performance is achieved at moderate acoustic intensities w

SUBMITTER: Nakajima K 

PROVIDER: S-EPMC12686939 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature