Proteome Analysis of Seven <i>Treponema pallidum</i> subsp. <i>pallidum</i> Strains Grown <i>In Vitr</i><i>o</i>.

ABSTRACT: Treponema pallidum subsp. pallidum (T. pallidum), the fastidious causative agent of syphilis, has become more accessible for research with its recently developed in vitro cultivation method. In this work, the proteomes of seven T. pallidum strains (Nichols-like: DAL-1, Haiti B, and Madras; SS14-like: SS14, Mexico A, Philadelphia 1, and Grady), cultivated in vitro, were analyzed in biological triplicates by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS/MS data were processed against their corresponding genomes using various annotation algorithms (DFAST, PGAP, Prodigal, Prokka, RAST, GeneMarkS, and manual GenBank annotation). Additionally, ORFfinder was used to predict all ORFs encoding polypeptides exceeding 50 amino acids. While

SUBMITTER: Bosak J 

PROVIDER: S-EPMC12687308 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature