Comparative Proteomic Profiling of Adrenocortical Neoplasia Using Mass Spectrometry.

ABSTRACT: Recent advances in high-throughput molecular analysis have significantly enhanced our understanding of the molecular mechanisms underlying adrenocortical diseases. To identify differences in protein signatures that may reveal insights into disease-specific pathogenesis, we used LC-MS/MS and bioinformatics to compare proteomic profiles of normal human adrenal (NHA) tissue, adrenocortical adenomas (ACA), adrenocortical carcinomas (ACC), and primary macronodular adrenocortical hyperplasia (PMAH) tumors, with and without ARMC5 mutations. In total, 7350 proteins were identified, and 3976 were quantified across all samples. Differentially expressed proteins (DEPs) were found in ACA vs NHA (27 DEPs), ACC vs NHA (49 DEPs), and PMAH vs NHA (81 DEPs). Comparing ACC and ACA revealed 64 upregul

SUBMITTER: Kremer JL 

PROVIDER: S-EPMC12687312 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature