Senescence-Induced Lipidome Alterations in Mesenchymal Stromal Cells.

ABSTRACT: Mesenchymal stromal cells (MSCs) show great promise as a clinical treatment for a variety of diseases, but their susceptibility to senescence during culture reduces the therapeutic potential and limits cell expansion. In this study, we explored how MSC lipid metabolism is altered in culture over time using ultrahigh-performance liquid chromatography mass spectrometry. The proportion of cells with senescence-associated β-galactosidase (SA-β-gal) activity was evaluated during 12 days of culture expansion of MSCs from two human donors. Lipid profiles were evaluated in parallel using exact mass and tandem mass spectrometry spectral database matching to generate 237 unique lipid annotations. Lipid abundance generally increased across most lipid classes over serial culture; however, many changes

SUBMITTER: Ogle ME 

PROVIDER: S-EPMC12687314 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature