Genotyping analysis of over 130,000 CIMMYT bread wheat breeding lines: A decade-long effort in optimizing wheat genotyping.

ABSTRACT: The International Maize and Wheat Improvement Center (CIMMYT) spring bread wheat (Triticum aestivum L.) program represents the largest and most diverse set of elite wheat germplasm globally. From 2013 to 2023, genotyping was conducted on 130,247 bread wheat lines advanced through CIMMYT's bread wheat breeding program with the objective to perform genomic prediction and identify genomic regions associated with important traits such as yield and disease resistance. We constructed and sequenced 636 genotyping-by-sequencing (GBS) libraries, multiplexed at 96- to 384-plex, and generated 30.7 terabases of sequence. Using an optimized TASSEL pipeline, we identified 24,125 high-quality single nucleotide polymorphism on 21 chromosomes. Population genetic clustering of 444 selected lines within 10 p

SUBMITTER: Shrestha S 

PROVIDER: S-EPMC12687407 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature