Tracing NAD⁺ metabolism uncovers adaptive coordination between host and microbiome during colitis.

ABSTRACT: Host-microbiota metabolic interactions critically regulate nicotinamide adenine dinucleotide (NAD⁺) homeostasis, and their disruption is increasingly linked to chronic diseases including inflammatory bowel disease (IBD). However, it remains unclear whether NAD⁺ dysregulation in IBD arises from impaired production, enhanced consumption, or both. Using multi-omics approaches and stable isotope-labeled NAD⁺ precursors administered via intravenous infusion in a murine model of dextran sulfate sodium (DSS)-induced colitis, we mapped tissue- and lumen-specific NAD⁺ metabolism under inflammatory stress. Our results reveal tissue-specific rewiring of NAD⁺ metabolism, with increased flux through the salvage pathway compensating for reduced d

SUBMITTER: Alsaadi AI 

PROVIDER: S-EPMC12687811 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature