Verbascoside targets endothelial HIF-1α/ Lysyl oxidase signaling to attenuate glomerular injury in diabetic nephropathy.

ABSTRACT:

Background

Diabetic nephropathy (DN) drives progressive renal fibrosis and functional decline, ultimately leading to end-stage renal disease. Pathological crosstalk between glomerular endothelial cells and mesangial cells is increasingly recognized as central to DN progression. However, whether endothelial-derived signaling specifically drives mesangial injury under diabetic conditions remains undefined.

Methods

We applied multi-omics profiling to identify pathogenic drivers. Target validation included qPCR and immunofluorescence co-localization in renal tissues. In vitro endothelial-mesangial crosstalk was modeled using conditioned media (CM) from mouse GECs applied to mesangial cells. Verbascoside (VB) was screened via structure-based virtual docking against LOX/LOX

SUBMITTER: Kang T 

PROVIDER: S-EPMC12687904 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature