Nuclear translocation of Cx43 promotes to CRC progression and associates with β-catenin accumulation.

ABSTRACT:

Background

The tumour microenvironment (TME) significantly influences intercellular communication, with several secreted factors activating both tumour cells and fibroblasts. Connexin43 (Cx43), a crucial gap junction protein, exhibits a significant regulatory role in tumourigenesis; however, the underlying regulatory mechanisms in colorectal cancer (CRC) are not fully understood.

Methods

Transwell co-culture system was utilized to evaluate fibroblast-mediated effects on CRC cells. Immunohistochemical analysis was conducted on clinical specimens. Cell migration and invasion capabilities were measured using Transwell assays. Subcellular localization was assessed via immunofluorescence. Protein interactions were validated by co-immunoprecipitation.

Results

The Wnt signal

SUBMITTER: Wang S 

PROVIDER: S-EPMC12688224 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature