A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer.

ABSTRACT:

Background

Prostate cancer (PCa) is a major health concern, and current PSA screening is limited by low specificity and the risk of overdiagnosis. Extracellular vesicle (EV)-derived RNA biomarkers offer a promising non-invasive alternative for early detection.

Methods

We utilized a tethered cationic lipoplex nanoparticle (TCLN) biochip for amplification-free EV RNA detection at the single-vesicle level. Eight candidate RNAs (four miRNAs, three mRNAs, and one lncRNA) were profiled in serum and urine samples from PCa patients, benign prostatic hyperplasia (BPH) patients, and healthy controls (HC). Diagnostic and risk stratification performance was evaluated in discovery and validation cohorts, with qRT-PCR used for validation.

Results

TCLN reliably detected the candidat

SUBMITTER: Diao Y 

PROVIDER: S-EPMC12688251 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature