Feasibility of establishing a nude mouse model of thymoma-associated myasthenia gravis.

ABSTRACT:

Background

The pathogenesis of thymoma-associated myasthenia gravis (TAMG) remains unclear, and one of the key problems is the lack of an appropriate animal model. The present study aimed to investigate the feasibility of establishing an animal model of TAMG.

Methods

Patient thymoma tissue specimens were transplanted into the left renal subcapsules of BALB/c nude mice under sterile conditions. The content of T lymphocytes and subsets, as well as total complement, in the mouse peripheral blood was detected using flow cytometry and enzyme-linked immunosorbent assay (ELISA) prior to and following transplantation. Furthermore, the tumor tissues were analyzed by immunohistochemistry (IHC).

Results

Transplanted thymoma tissues survived in the mice with a success rate of 61%

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12688567 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature