Identification of lysozyme in Venetin-1 nanoparticle from the coelomic fluid of the earthworm <i>Dendrobaena veneta</i>.

ABSTRACT:

Background

In the present study, lysozyme was analyzed in the nanoparticle Venetin-1 obtained from the coelomic fluid (CF) of the earthworm Dendrobaena veneta. Venetin-1 is a protein-polysaccharide complex and has proven anti-cancer (non-small lung cancer, colon cancer, cervical cancer), antifungal and immunostimulating properties.

Methods

The studies were conducted using methods such as electrophoretic separation, immunodetection, and ELISA determination of lysozyme concentration. Spectroscopic methods such as FTIR and Raman spectroscopy were also employed. Proteomic analyses, SageELF separation, and zeta potential determination were also performed. Cryo-EM was used to determine the molecular structure of Venetin-1.

Results

The lysozyme-type activity in this c

SUBMITTER: Fiolka MJ 

PROVIDER: S-EPMC12689324 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature