LL37-driven mast cell degranulation and inflammation in rosacea via TLR2/JAK2/STAT3 axis.

ABSTRACT:

Introduction

Rosacea is a chronic inflammatory facial dermatosis with incompletely elucidated pathogenesis. LL37 is a key molecular mediator in rosacea development, and mast cells represent pivotal immune players in this process. However, the precise mechanism underlying LL37-induced mast cell degranulation remains undefined.

Methods

A LL37-induced rosacea-like dermatitis mouse model was established with or without ruxolitinib treatment. Hematoxylin-eosin and toluidine blue staining were used to evaluate the pathological changes. Mice skin lesions were collected for transcriptome sequencing, Western blot and immunofluorescence. In vitro, the interaction between LL37 and TLR2 on the mast cell membrane surface was detected by co-immunoprecipitation and fluorescence staining. T

SUBMITTER: Fan H 

PROVIDER: S-EPMC12689332 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature