Neural stem cell transplantation attenuates cognitive decline and neuroinflammation in a mouse model of Alzheimer's disease with chronic cerebral hypoperfusion.

ABSTRACT: Alzheimer's disease (AD) and chronic cerebral hypoperfusion (CCH) frequently coexist in aging populations, synergistically aggravating neurodegeneration. To assess the therapeutic potential of stem cell interventions, an AD + CCH mouse model was generated by combining APP/PS1 mice with bilateral common carotid artery stenosis. Neural stem cells (NSCs) or induced pluripotent stem cells (iPSCs) were transplanted into the lateral ventricles at 5 months of age. Behavioral testing, Nissl staining, Western blotting, and immunofluorescence were conducted at 9 and 12 months to evaluate cognition, neuronal survival, cell death pathways (LC3-II, cleaved caspase-3, NLRP3), glial polarization, and neurotrophic/synaptic markers (BDNF, VEGF, VAChT, PSD95). CCH exacerbated AD-related cognitive deficits,

SUBMITTER: Lu Z 

PROVIDER: S-EPMC12689354 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature