Minimally expanded breast cancer tumor-infiltrating-lymphocytes provide guidance for therapeutic selection.

ABSTRACT:

Introduction

The analysis of tumor-infiltrating lymphocytes often requires techniques that expand their numbers, potentially introducing bias. To address this, we performed a detailed analysis of minimally cultured TILs to evaluate whether this approach better preserves their characteristics.

Methods

The TIL culture method was based solely on tumor tissue with low IL-2 supplementation to minimize artificial alterations. The validity of this approach was confirmed by the correlation between CD3+ T cell percentages in cultures and infiltration patterns observed by immunohistochemistry. Immunophenotyping, cytokine release, and TCR repertoire analysis were used to characterize CD4+ and CD8+ T cell subsets and their molecular features during minimal expansions.

Results

Hig

SUBMITTER: Aran A 

PROVIDER: S-EPMC12689370 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature