Cross-species validation of a 6-miRNA blood signature for Parkinson's disease: from MPTP mice to human PBMC and serum exosomes.

ABSTRACT:

Introduction

Early detection of Parkinson's disease (PD) remains challenging due to the lack of reliable blood-based biomarkers. While microRNAs (miRNAs) show promise as circulating biomarkers, translating preclinical discoveries to clinically applicable panels requires rigorous validation across platforms and populations.

Methods

We performed temporal miRNA profiling in an acute MPTP mouse model (day 0 vs. day 5, n = 4 per group) using limma differential expression analysis with FDR correction. To address high-dimensional small-sample challenges, we employed global permutation testing and stability selection with elastic net regularization over 2,000 iterations. A compact miRNA panel was derived and validated in three independent human cohorts: GSE16658 (PBMC, n</

SUBMITTER: Jung N 

PROVIDER: S-EPMC12689404 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature