An innovative infection method for the accumulation of viral nanoparticles in <i>Nicotiana benthamiana</i>.

ABSTRACT: Tomato Bushy Stunt Virus (TBSV) naturally infects tomato plants, although it can also infect other plant species, such as Nicotiana benthamiana, a known model system in plant molecular farming. In the presented work, a novel system for TBSV infection of Nicotiana benthamiana plants, designed to produce nanomaterials, was developed and optimized based on a simple foliar spray, without the use of surfactants. Up to now, the standard procedures for the viral infection have been syringe or vacuum infiltration, which are a time-consuming manual procedure or requiring expensive machinery, respectively. The spraying method was chosen because it could be implemented in industrial conditions, such as vertical farms, where spraying systems are already present or can be easily installed

SUBMITTER: Ljumovic K 

PROVIDER: S-EPMC12689547 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature