Comprehensive metabolites characterization of Alpinia katsumadai seeds via a multiplex approach of UHPLC-MS/MS and GC-MS techniques.

ABSTRACT: Alpinia katsumadai Hayata (Zingiberaceae) is an edible seed valued in traditional medicine for its remarkable therapeutic properties. The seed serves as a fragrant culinary spice, adding a unique flavor to various foods. In this study, three approaches were employed to elucidate seed metabolome including UHPLC-MS/MS-based molecular networking, HS-SPME/GC-MS, and post-silylation GC-MS. A total of 82 secondary metabolites spanning diverse chemical classes were annotated, including the report of two newly annotated catechin-guibourtinidol derivatives and deoxy phloretin-3'-C-hexoside for the first time in Alpinia genus, alongside the first hypothesis of calyxins' class mass fragmentation pattern. HS-SPME/GC-MS analysis led to the detection of 30 aroma determinants dominated by sesquiterpenes

SUBMITTER: Ali NB 

PROVIDER: S-EPMC12689793 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature