MicroRNA identification and expression analysis of wheat thermo-sensitive male sterile line BNS366 for fertility transformation.

ABSTRACT:

Introduction

Thermo-sensitive male sterile lines are the key element in the two-line hybrid system of wheat; some specific microRNAs (miRNAs) are involved in the development of anthers in plants, being an important cause of male sterility. Bai-Nong Sterility 366 (BNS366) is an excellent material for the study of thermo-sensitive male sterility, but no information is available on the role of miRNAs in regulating fertility in BNS366.

Methods

In this study, miRNAs in the pollen mother cell periods and the tetrad periods of the low-temperature sterile and normal fertile anthers of BNS366 were characterized using RNA sequencing (RNA-seq).

Results

MiRNA sequencing identified 22 differentially expressed known miRNAs and eight novel miRNAs with the largest differential expres

SUBMITTER: Chen J 

PROVIDER: S-EPMC12689989 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature